Recombinant vector of pUCm-T/mIA-2i-mIgG Fc was prepared by gene combination and confirmed by PCR and dual-site endonuclease.

• 获得重组克隆质粒pUCm-T/mIA-2i-mIgGFc,经聚合酶链反应和双位点酶切鉴定重组成功。