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- The inhibition effect of BACE siRNA on BACE expression was investigated by fluoroscopy and immunohistochemistry method. 制备稳定高表达BACE基因的神经母细胞瘤SK-N-SH细胞株,用荧光显微镜和免疫组织化学的方法观察siRNA对BACE表达的影响。
- Positional and time effects of specific siRNA inhibit the expression of h TERT... 特异性siRNA抑制hTERT基因表达的位置及时间效应 Positional and time...
- The sequences of RIP2 siRNA expressing plamid we had constructed was concordant with what we had designed. 2. 结果 1、构建的RIP2 siRNA表达质粒的序列与我们设计的一致。
- Administration of ephrinB2 siRNA reduced the expression of ephrinB2 and mechanical allodynia. ephrinB2siRNA干预可降低ephrinB2的表达和机械性异常疼痛的强度。
- Objective To construct Sox9 siRNA expression vector in order to make clear the role of Sox9 in the genesis of chondrosarcoma. 目的构建Sox9siRNA表达载体,分析Sox9在软骨肉瘤中的作用机制。
- Objective: To investigate the effects of siRNA against survivin combined with tamoxifen on the proliferation and apoptosis in breast cancer MCF-7 cell. 目的:观察靶向survivin的siRNA联合三苯氧胺对乳腺癌MCF-7细胞增殖和凋亡的影响。
- The ratios of COX-2 mRNA in NC, 1#-4#siRNA or HeLa group to CTL group were 0.72, 0.3, 0.25, 0.4, 0.04, 2.1 respectively by semi-quantitative RT-PCR. 转染36h;PCR结果显示;CTL、NC、1%23siRNA、2%23RNA、3%23RNA、4%23siRNA、阳性对照HeLa细胞组hCOX-2 mRNA电泳条带密度值分别为1、0.;72、0
- Another role of siRNA is directing RITS complex to bind with homologue chromosome, and then induces heterochromatinization. 除了与RISC结合外,siRNA还引导了RITS复合物结合到同源染色质,介导异染色质化过程。
- The scientists made versions of siRNA that blocked the forms of PCSK9 found in mice, rats, nonhuman primates and humans. 科学家们制作了多种版本的siRNA,可以阻止小鼠、大鼠、非人类的灵长类和人类中各种形式的PCSK9蛋白表达。
- Previous siRNA trackers gave off light for less than a minute, while quantum dots, developed for imaging, emit light for hours at a time. 不过,量子点为何比现有技术更加有效,其原因还是个谜。
- Fourth, miRNA sequences are nearly always conserved in related organisms, whereas endogenous siRNA sequences are rarely conserved. 第四,相关生物中的微小RNA序列几乎总是保守的,而外源小型干扰RNA 序列很少是保守。
- Sequence-specific siRNA (OPNi-2) targeting OPN can significantly reduce OPN mRNA expression in HCC-LM3 cell line with highly metastatic potential. OPNi-2可以特异性抑制高转移肝癌细胞系(HCC-LM3)内OPN的表达。
- And LipofectamineTM 2000 mediated gene transfection of p498 siRNA expression vector into COS-7 cells downregulated the mRNA and protein level of 4-1BB at 48h after transfection. 结果经测序分析;各m4-1BB siRNA表达载体构建成功; 其中p498 siRNA与p4-1BB双质粒转染COS-7细胞;48h后可观察到细胞表面4-1BB分子明显下调;其胞内mRNA水平也显著降低;与无关干扰组比较;差异有统计学意义(P<0.;05)。
- The data of FCM as well as Fluorescence Dyeing assays demonstrated that siRNA targeting CSB could obviously increased apoptosis induced by UV irradiation. 荧光染色结果亦说明siRNA可促进细胞凋亡的发生并提高其对紫外线(UV)照射的敏感性。
- Construction of Tim-1 siRNA expression vectors and its inhibitory effect on Tim-IHA expressionFour pairs of oligonucleotide targeting to Tim-1were designed and synthesized. 针对Tim-1的siRNA表达载体的构建及其对Tim-1HA的抑制效果
- CONCLUSION: Down regulation of MAD2 expression of SGC7901 bv sequence-specific siRNA could accelerate the cell growth and impair the mitosis arrest of SGC7901 induced by VCR. MAD2的抑制可使胃癌细胞SGC7901生长加速,增殖能力增强,同时减弱纺锤体抑制剂长春新碱阻滞细胞周期的作用。
- Effects of siRNA targeting CD147 gene on the expression of CD147 and the proliferation of malignant melanoma cell line A375[J].Chin J Dermatol,2006,39 (4):204-206. CD147 siRNA对恶性黑色素瘤细胞株A375增殖和CD147表达的影响[J].;中华皮肤科杂志;2006;39(4):204-206