RT-PCR and real-time quantitative PCR were performed to detect ORF26 mRNA expression in heterokaryons that were created by BCBL-1 cells fused with HHV-6-infected JJhan cells (also known as HV6-J).

 
  • 本研究将HHV-6 U1102株感染的JJhan细胞与HHV-8潜伏感染的BCBL-1细胞进行细胞融合,采用RT-PCR和RealTime RT-PCR对HHV-8裂解周期基因ORF26的转录水平进行检测。
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