RT PCR result showed that after 4h,12h,24h incubation respectively, 30% supernatant can not increased insulin messenger RNA levels significantly at the present of 16.7mmol/L glucose compared with INS 1 cells cultured with glucose alone.

• RT PCR结果显示 ,在 1 6 7mmol L葡萄糖刺激 4、1 2和 2 4h后 ,30%25上清液对INS 1细胞胰岛素mRNA水平均无明显影响。