Qi319 (Pi), which is highly resistant to southern rust, and 5 inbred lines 478, 340, 9801, Luyuan92, Huangzao4 (P2), which are all highly susceptible to southern rust, were crossed respectively.

• 南方锈病高抗自交系齐319分别与高感自交系鲁原92、黄早4、478、340、9801杂交(F_1)，F_1自交(F_1)及与感病亲本回交(BC_F_1)。