Pulmonary microvascular permeability was evaluated by Evans blue dye concentration. Intestinal injury was assessed by histological method. The expression of pulmonary NOS (iNOS and eNOS) and its?

• Evans蓝法测定大鼠肺毛细血管通透性、逆转录 聚合酶链式反应 (RT PCR)法测定肺NOSmRNA表达、Western印迹法测定肺组织NOS表达 ,同时进行小肠组织学检查以评价肠损伤程度。