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- AIM: To construct pET32a/His MBL-CLR recombinant prokaryotic expression plasmid and to express mannan-binding lectin-CLR (MBL-CLR) protein in E. 目的:在大肠杆菌中表达人甘露聚糖结合凝集素(MBL)胶原样区(CLR)蛋白。
- Methods:The epf gene fragment was amplified by PCR from ZYH24 and cloned into prokaryotic expression plasmid pGEX4T-2 to form pGEX4T-2-epf. 方法根据GenBank S.;suis2epf基因序列设计引物;克隆ZYH24株epf基因片段并进行序列分析;
- Then the BLY cDNA fragment was subcloned into prokaryotic expression vector pGEX-4T-1, forming the prokaryotic expression plasmid (pG-BLY). 克隆PCR产物，并构建了pGEX-4T-1-BLY原核表达载体。 经BamHI和EcoRI酶切及质粒PCR鉴定，证实本实验构建的新型牛溶菌酶基因已克隆到原核表达载体pGEX-4T-1上，为进一步研究其诱导表达条件及生物学功能奠定了基础。
- Methods:(CTP)_4coding gene from pBSMR-(CTP)_4 was cloned into prokaryotic expression vector pET-28a(+) and the expression plasmid pET-28a(+)- (CTP)_4was obtained. 方法:将pBSMR-(CTP)_4中的(CTP)_4基因片断克隆入表达载体pET-28a(+),得到表达质粒pET-28a(+)-(CTP)_4。
- The identified cDNA was cloned into the new type of prokaryotic expression vector pQE-80L, and a synthetized expression plasmid named pQE-80L/DHFR/ABP obtained. The E. 将此基因克隆到原核表达载体pQE-80L,获得融合表达质粒pQE-80L/DHFR/ABP,在1%25IPTG诱导下进行表达。
- Then, a constructed prokaryotic expression plasmid of pGEX-2T-E7 presented by Dr. Werner, was transformed into E. coli BL21(DE3 stain) and induced by IPTG to express GST-E7 fusion protein. 为了研究HPV16E7疫苗的需要，我们利用Werner博士惠赠的pGEX-2T-E7原核表达质粒转化入大肠扪：菌BL21(DE3)中，用IPTG诱导后，裂解细胞，用SDS-PAGE鉴定诱导效果，发现在43kDa处有融合蛋白的表达。
- Objective To construct the prokaryotic expression plasmid expressing woodchuck hepatitis virus core antigen(WHcAg) and prepare polyclonal antibodies. 目的构建土拨鼠肝炎病毒核心蛋白质粒并进行原核表达、抗体制备。
- Objective: The aim of this study is to construct a prokaryotic expression plasmid of cDNA sequence encoding first 193 amino acids of BPI N-terminal and to express it in E.coli BL21. 目的：建立表达BPI N-端193个氨基酸的重组表达质粒，获得BPI193重组蛋白，为进一步科研实验和临床治疗创造条件。
- A prokaryotic expression plasmid was constructed by inserting the BC-48 gene into pGEX-4T-2 vector. Expressed protein induced by IPTG was analyzed by SDS-PAGE and Western-blotting. 构建BC-48的重组pGEX-4T-2表达载体,经IPTG诱导表达后,进行SDS-PAGE、Western-blotting分析。
- The prokaryotic expression plasmid pET-32a/NAP 5 was constructed successfully. The recombinant was transformed into E. coli BL21(DE3) and expressed by inducing with IPTG and lactose with high efficiency. 成功构建了pET-32a/NAP5表达载体,IPTG和乳糖均能诱导目的蛋白在大肠杆菌BL21(DE3)中高效地可溶性表达。
- The prokaryotic expression plasmid pGEX-IL-24 was constructed,GST-IL-24 molecular mass was approximately 50 000.Conclusion GST-IL-24 fusion protein was expressed in E. coli BL21(DE3) which was transformed from the recombinant vector of IL-24. 结论IL-24的重组载体在大肠杆菌BL21(DE3)可以表达GST-IL-24融合蛋白。
- prokaryotic expression plasmid recombinant 原核表达质粒
- Construction of prokaryotic expression plasmid ET15b-PEP-1-SOD1 原核表达质粒pET15b-PEP-1-SOD1的构建
- In order to study the function of HSF, two human HSF prokaryotic expression plasmids ( pET-11/HSF1 and pET-11B/HSF2 ) were constructed and expressed efficiently in E. 建立了两种人HSF原核表达体系并研究了原核表达产物与顺式作用元件HSE(heat shock element)相互作用的性质。
- Construction and expression of prokaryotic expression plasmid of cagA gene of coccoid Helicobacter pylori 球形幽门螺杆菌cagA基因原核表达质粒的构建及表达
- Construction of Prokaryotic Expression Plasmid of the Human Tumor Suppressor Gene PTEN and Its Expression in E. Coli 人抑癌基因PTEN的原核表达载体的构建及融合表达
- Construction of the recombinant prokaryotic expression plasmid contain BAG domain in Arabidopsis thaliana 编码拟南芥含BAG结构域蛋白的原核表达载体构建
- Keywords phenylketonuria;phenylalanine hydroxylase;clone;prokaryotic expression plasmid; 苯丙酮尿症;苯丙氨酸羟化酶;克隆;原核表达质粒;
- The construction of prokaryotic expression plasmid and high-level expression of the human single chain Fv antibody fragment gene to rabies virus 人源抗狂犬病毒单链抗体基因原核表达质粒的构建及高效表达
- Construction of the Recombinant Prokaryotic Expression Plasmid pET-21a (+) -DCN and the Influences on BGC-823 Gastric Cancer Cell Line DCN原核表达体系的构建及其对胃癌细胞株BGC-823的影响
