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- In the present study, a female-specific AFLP marker was isolated from Cynoglossus semilaevis by AFLP technique using the selective primer combination E-ACT/M-CAA. 文章采用AFLP技术, 利用选择性引物组合(E-ACT/M-CAA)从半滑舌鳎中筛选到一条雌性特异的AFLP标记。
- Exception of the combination E-AGC/M-CAA,all primer combinations had not detected polymorphism between the two pools. 分析表明,64组引物在两池间全部扩增出了稳定、清晰的带纹,共计3477条带。
- Selective amplification primer combination patterns EcoRI+2/Msel+3 , EcoRI+3/MseI+3 were both suitable to polymorphism analysis of peach genome. The result showed that the AFLP system was can be used for the genome of peach. 籽预扩增产物稀释20倍用作选择性扩增的模板,选用EcoRI+2/MseI+3、EcoRI+3/MseI+3所组成的128对引物组合进行扩增,扩增条带清晰、多态性高,适合桃基因组分析。
- Primer combination E+AAC/M+CTT amplified five paternal characteristic bands between Slhy353 and Blho301:440 bp,393 bp,289 bp,162 bp and 67 bp,whereas E+ACA/M+CAT generated four characteristic bands:448 bp,360 bp,217 bp and 145 bp. 引物对E+AAC/M+CTT在亲本 Slhy353×Blho301 间扩增出 5 条父本特征带:440 bp,393 bp,289 bp,162 bp和67 bp,引物对 E+ ACA/M+ CAT 则产生了 4 条: 448 bp, 360 bp, 217 bp和 145 bp;
- Results 15 primer combinations were screened from 64 primer combinations to analysis DNA polymorphism and the DNA fingerprinting were generated by primer combination E-AGG/M-CAA. 结果 从64对引物组合中筛选出了15对引物组合进行多态性分析,以多态位点百分率最高的E-AGG/M-CAA组合构建甘草属五种植物不同来源的指纹图谱。
- Using 28 selected primer combinations, 12 accessions of Bendizao tangerine from seven provinces were investigated by AFLP analysis. 摘要采用AFLP分子标记对全国7省的12份本地早橘资源进行遗传多样性分析。
- SRAP assay was conducted using 10 peanut cultivars from China of 3 market types and 51 SRAP primer combinations to evaluate the utility of SARP in peanut. 研究了分属3个市场型的10个中国花生栽培种的序列相关扩增多态性(SRAP)。结果表明,SARP技术可以揭示花生栽培种的遗传差异。
- The primer combinations by which more polymorphic markers (showed in brackets) were obtained involved in E00+ACG/M00+CAA (13), E00+ACG/M00+CAG (10), E00+AAC/M00+CAC (8) and E00+AAC/M00+ACT (7). 多态标记数较多的引物组合为 :E0 0 +ACG/M 0 0 +CAA(13个 )、E0 0 +ACG/M0 0 +CAG(10个 )、E0 0 +AAC/M0 0 +CAC(8个 )和E0 0 +AAC/M0 0 +ACT(7个 )。
- Genetic diversity of 11 Acer species was analyzed by AFLPs. Five pairs of primer with high polymorphism and powerful distinctiveness were selected from 12 ones. Three AFLP primer combinations revealed 101,163,178 polymorph bands respectively. 采用了AFLP技术分析了槭属十一种植物的遗传多样性,从12对AFLP引物中筛选出5对多态性高、分辨率清晰的引物,其中选择最优的3个引物组合分别扩增出101、163、178条多态性带。
- Twenty-one AFLP primer combinations of PstlI/Msel were used in the study and 641 AFLP amplified bands were obtained, including 586 polymorphic bands with an average of 30.52 per primer combination. The polymorphism percentage was 91.4%. 结果表明21个PstI/MseI引物组合检出可辨认带共641条,其中具多态性带586条,平均每对引物得到30.;52条多态性带,多态性91
- The results showed that 3986 loci of AFLP markers were amplified with 24 suitable primer combinations, 3546 loci were polymorphic, and the percentage of polymorphism loci reached 88.96%. 结果表明:用筛选出的24对适宜引物共扩增出3986个AFLP位点,其中多态性位点3546个,多态性位点比率高达88.;96%25;
- I borrowed from the library a primer of phonetics. 我从图书馆借了一本语音学入门书。
- Two specific fragments linked to recessive d location of D/d gene derived from primers combination E-TA/M-CTC and E-AT/M-CTA, the linkage distance were 1.47cM and 2.99cM respectively. 其中与D/d基因隐性位点紧密连锁的两个标记,分别来自引物组合E-TA/M-CTA、E-AT/M-CTA,连锁距离分别为1.;47cM,2
- Many anchored microsatellite primers were developed for RAMP, and more primer combinations could be obtained by combining with different RAPD primers. 目前已开发出多个RAMP锚定微卫星序列引物,与不同的RAPD引物进行扩增,得到更多的引物组合。
- We tried it in every conceivable combination. 我们把能想到的各种组合都试了一遍。
- These players made a very good combination. 这些球员们配合得很好。
- He had forgotten the combination of the safe. 他忘记了保险箱密码。
- The 20 microsatellite markers and 6 AFLP primers combinations were used to detect genetic variation of 19 Chinese Native chicken breeds. 摘要利用20个微卫星标记和6对AFLP引物组合对19个地方鸡种进行了遗传检测,并根据两种标记分析的遗传距离进行了聚类分析。
- A combination of two or more elements or parts. 复合物由两种或多种元素或部分构成的混合物
- The harmonic combination of these tones. 四度和音音调的和谐组合