Preparative high-speed counter-current chromatography (HSCCC) was used for the separation of calycosin and formononetin from the ethyl acetate extract of Astragalus membranaceus Bge.

• 采用高速逆流色谱法(HSCCC),以正己烷氯仿甲醇水组成二相系统作为固定相与流动相,对黄芪的乙酸乙酯粗提物进行了分离纯化。