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- Thin recombinant with the cDNA ~crt fragment was released frc the positive clone and was di ?z; tcJ with EcoRI; the eDNA fra, ent was 2kb in length. 释放质粒后,进行酶切鉴定,该阳性克隆cDNA插入片段的大小为2kb左右。
- Blast analysis displayed one of the Northern blot positive clone is homologous with autotaxin gene, and another is homologous with calcyclin gene. 对这13个克隆进行测序和Blast同源序列分析表明,其中一个Northern杂交阳性克隆与autotaxin基因同源,另一个与钙周期蛋白基因同源。
- Methods:Screening peptide library with EAN serum. Identified positive clone by ELISA and compared similarity between positive clone and LPS of Campylobacter jejuni. 方法 :采用噬菌体呈现技术对 8个EAN模型血清抗体进行肽库筛选 ,并将获得的克隆用ELISA方法进行阳性鉴定 ,以及将阳性克隆与空肠弯曲菌LPS相似性进行比较。
- The recombinant plasmid was transformed into the yeast strain S. cerevisiae BJ1990 to carry out the primary expression experiment. In culture supernant of screened positive clone, the hirudin activity was detected to be 30 ATU/ml. 用正确的重组表达质粒转入酿酒酵母BJ1990细胞进行了初步表达实验,在筛选出的阳性克隆的培养上清中检测出的水蛭素活性为30ATU/ml,表达量达4mg/L。
- MBD1 siRNAs had beensuccessfully integrated into the plasmid. MBD1 siRNAs vector was transfected intopancreatic cancer cell AsPC-1 by liposome. Positive clone was obtained by the screenof G-418. 采用脂 质 体 介 导 的 方 法 将 MBD1siRNAs 表 达 质 粒 转 染 胰 腺 癌 细 胞 系AsPC-1,通过 G-418 的筛选获得稳定表达 MBD1siRNAs 的阳性克隆。
- In this study, the dicistronic expression vector (pEGFP-C1) was used to be transfected into human lung cancer cell line(ASTC-a-1)and a positive clone which stably expressed GFP in high level was obtained. 利用常规转染方法将带有EGFP基因的质粒载体导入人肺腺癌肿瘤细胞 (ASTC a 1) ,并得到GFP稳定表达的细胞株。
- The obtained bands were cloned into pGEM T-Easy vector, and the positive clone were confirmed by EcoR I enzyme digestion. 将所获片段克隆入pGEN T-Easy载体,EcoR I酶切鉴定。
- There is positive proof that he did it. 有确切的证据证明他做了此事。
- AL01 library contained 23 650 positive clones and the av-erage foreign DNA fragments were about 3.2 kb. AL01文库包含23650个克隆;随机插入载体的外源DNA 片段平均大小为3.;2Kb 左右;DNA 文库的总容量为75
- The obtained 30 positive clones were all sequenced, and analyzed by BLAST (basic local alignment search tool). 将得到的30个阳性克隆全部进行测序,并进行BLAST同源序列比对分析。
- In our studying, we not only used the CAPC data in positional clone, but also developed about 30 novel makers based on CAPC and new protocol of heterogeneous double helix analysis for SNPs or small InDels. 我们不仅利用CAPC数据资源进行定位,而且还发展了近30个新分子标记,在分子标记的检测方面发展了异源双螺旋分析技术。
- Conclusion The identification of positive clones provides a possible way for the development of toxoplasmosis vaccine. 结论阳性克隆的筛选和鉴定为抗弓形虫病疫苗的研制提供又一途径。
- SPV Vp2 protein was successfully expressed by the positive clones under the induction of IPTG. (2)首先用大肠杆菌表达和部分纯化的SPV VP2融合蛋白建立的Western印迹分析检测了猴子暴露者中的血清SPV抗体,同时用检测人细小病毒B19 IgG抗体的商品试剂检测细小病毒抗体以排除可能存在的B19的交叉抗体反应。
- The PCR products were cloned into T clone vector,and the positive clones were picked out,then the T clone vector was identified by restriction endonuclease digestion. 将扩增的连接产物克隆入T载体,挑选阳性克隆并进行酶切鉴定,酶切鉴定正确的克隆进行拼接产物的核苷酸序列测序。
- As the most efficient strategy in gene clone, positional cloning has been used widely in QTL cloning in rice. 图位克隆作为基因克隆的最有效手段, 在水稻QTL克隆方面取得了很大的进展。
- By sequence alignment, 109 special microsatellite positive clones were confirmed finally, which contained 179 microsatellite DNA domains. 序列比对后最终获得109个具有特异微卫星序列的阳性克隆,其中包含179个微卫星DNA结构域。
- Results: After 4 rounds of panning,20 positive clones were obtained and 18 clones were found to be able to bind keratin antigen specifically. 结果: 经过4轮筛选,获得20个能与角蛋白结合的阳性克隆,其中可产生特异性抗角蛋白抗体的克隆18个。
- The resu1ts of MCT1 gene ani-sense reconsmicted vector transfectionand identification: after being transfected, A549 cells were screened byG4l$,and positive cloning was obtained. (1)MCT1基因反义重组载体转染、鉴定分析结果:反义基因转染A549细胞后,经G418筛选,出现阳性克隆;
- Methods Newborn larvae(NBL) cDNA library of Trichinella spiralis was screened using the infected swine serum and positive clones were sequenced and analysed. 方法 以旋毛虫感染猪血清为抗体探针 ,对旋毛虫新生幼虫cDNA文库进行免疫筛选 ,将获得的阳性克隆进行测序及分析。
- The library was screened by the RGA probe of Hvrgak4. Four positive clones were obtained among 1.2 X 106 clones in the library through two rounds southern hybridization. 从cDNA文库的1.;2×10~6个克隆中经两次筛选获得四个阳性克隆:kong1插入片段2