Porcine parvovirus(PPV) was cultivated on IBRS-2 cell, after CPE was observed, the PPV was harvested, frozen and thawed, then inactivated with Formalin, precipitated with (NH4 )2S4., finally dialysed and concentrated.

• 将猪细小病毒在IBRS-2细胞上同步培养增殖，待出现细胞病变后，反复冻融，收获病毒液，用甲醛灭活。