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- Isolation and identification of a Bifidobacterium strain with a plasmid polymerase by PCR 一株双歧杆菌质粒聚合酶基因的PCR扩增和鉴定
- Plasmid polymerase 质粒聚合酶
- Full-length plasmid DNA was amplified with the above primers with a high-fidelity thermostable DNA polymerase capable of long-rang amplification. 用能扩增长片段的高保真耐热 DNA聚合酶扩增全长的质粒DNA,直接转化大肠杆菌。
- Methods Variation of P gene of HBV polymerase was determined by short segment PCR ELISA according to the recombined plasmid which was acquired by the site directed mutagensis. 方法 通过定点诱变获得的重组质粒作为标准对照 ,采用短片段PCR ELISA方法对拉米呋啶治疗的 6 0例乙肝患者血清标本进行HBV聚合酶P基因的变异检测 ,同时观察其HBVDNA定量、肝功能指标的变化。
- The plasmid pBV220-Arr. could be expressed in E. 成功构建的重组质粒pBV220-Arr在E.
- FastStart Taq DNA Polymerase, December ed. 酸外切酶活性( Roche Package Insert.
- The recombinant plasmid pUC18 E6 had been got. 获得重组质粒 pUC18 E6。
- Specifically inhibits chloroplast RNA polymerase. 特异性的抑制叶绿体RNA聚合酶。
- METHODS: Human arresten gene was amplified from recombinant plasmid pGEM-Arr with polymerase chain reaction (PCR), and then cloned into prokaryotic expression vector pRSET by means of recombinant gene technology. 方法 :利用聚合酶链式反应 (PCR) ,由我们构建的重组质粒pGEM -Arr中扩增出arresten基因 ;
- For his invention of the polymerase chain reaction (PCR) method. (聚合酶链反应法的发明)。
- Recombinant plasmid pGEX-Csn was transformed into E. 重组质粒pGEX-Csn转化E.
- Objective To construct the plasmid expressing BDV p40 gene. 目的构建博尔纳病病毒p40基因重组表达质粒。
- In addition, a set of expression plasmid vectors, PMS-31b. 同时构建一组质粒表达载体PMS-31b。
- Constructed the expression plasmid pTrc-rCR and checked it. 构建表达质粒pTrc-rCR,并酶切检验;
- The PNL-BDNF-IRES2-EGFP plasmid was identified to be correct. 构建的慢病毒载体质粒PNL-BDNF-IRES2-EGFP经Sal I和BamH I双酶切鉴定正确,三质粒共转染293T细胞后荧光激发可见大量绿色荧光。
- In the 6th week plasmid DNA could only be detected in quadriceps. 第6周除在股四头肌可检测到外,其余组织中均为阴性;
- I focus on the role of antisense RNAs in plasmid replication. 本文着重叙述反义RNA在质粒复制中的作用及其进展。
- Polymerase chain reaction-single strand conformation polymorphism, PCR-SSCP. 目的:探讨聚合酶链反应-单链构象多态性分析。
- Methods To use specific serosorbernt templat polymerase chain reaction(SSST. 方法应用特异血清吸附模板聚合酶链反应(SSST。
- Adult hen (white leghorn) was immunized with 615 mouse liver RNA polymerase B. 摘要 用615小鼠肝RNA聚合酶B免疫母鸡获得了抗血清。