Peripheral blood mononuclear cells(PBMNC)were incubated with phytohemagglutinin(PHA). Before or after T cell activation,the cells were cultured with or without rhCD25MAb or cyclosporine A(CsA)in vitro.

• 应用植物血凝素(PHA)刺激外周血单个核细胞,不加或同时加rhCD25MAb或环孢菌素A(CsA)进行体外培养,或先用PHA刺激T细胞活化后再加入rhCD25MAb继续进行培养。