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- Localization of activity of immobilized penicillin G acylase was studied by X ray microanalysis. 利用X射线微区分析,对固定化青霉素G酰化酶的活性进行了定位分析。
- Methods:The influence of ammonium sulfate and glucose on plasmid stability and penicillin G acylase activity were conducted in flask shakes and a 5L fermentor. 发酵过程中维持低葡萄糖水平可以限制细胞的生长速率,提高质粒稳定性和促进青霉素G酰化酶的合成。
- The glucose concentration was controlled around 0.1g/L and the average specific growth rate was 0.06h~(-1). Under the above conditions the plasmid stability reached 86% and penicillin G acylase activity was 23 000U/L. h恒速流加葡萄糖至35h;控制流加过程葡萄糖浓度0.;1g/L左右;平均比生长速率为0
- immobilized penicillin G acylase 固定化青霉素酰化酶
- STUDY ON CONVERSION OF 7-ADCA WITH IMMOBILIZED PENICILLIN G ACYLASE 头孢G酸酶裂解制备7-ADCA的研究
- Site-directed Mutagenesis at Ser177 of Penicillin G Acylase Gene 青霉素G酰化酶基因Ser177的定点突变
- Keywords Mesoporous materials;Penicillin G acylase;Stability; 介孔材料;青霉素酰化酶;稳定性;
- Keywords penicillin G acylase;Bacillus megaterium;phage display; 关键词青霉素G酰化酶;巨大芽孢杆菌;噬菌体展示;
- The Purification and Properties of Penicillin G Acylase From E.coli AE109 大肠杆菌AE109青霉素G酰化酶的分离纯化及性质研究
- Keywords SEM photograph;immobilized penicillin G acylase;EDS maps;activity; 扫描电镜;固定化青霉素G酰化酶;能谱仪面分布;活性;
- WHOLE NUCLEOTIDE SEQUENCE OF PENICILLIN G ACYLASE GENE AND ITS FLANKING REGION FROM E.COLI 大肠杆菌青霉素G酰化酶基因及其邻近区域的核苷酸全序列
- The Molecular Rebuilding of Penicillin G Acylase and the Expression of Recombinant Sars Cornavirus Proteins 青霉素G酰化酶的分子改造及重组SARS冠状病毒蛋白质的表达
- Keywords catalytic reaction mechanism;penicillin G acylase;kinetic method;ionizing residues; 催化反应机制;青霉素G酰化酶;动力学方法;离子化基团;
- Keywords Hydrophilic magnetic polymer;Oxirane;Inverse suspension polymerization;Penicillin G acylase;Immobilization; 亲水性磁性聚合物;环氧基;反相悬浮聚合;青霉素酰化酶;固定化;
- Keywords X-ray microanalysis;capture reagent;immobilized penicillin G acylase;activity; 射线微区分析;捕捉剂;固定化青霉素G酰化酶;活性;
- Keywords 7-ADCA;fluidized bed reactor;fixed-bed reactor;synthesization;kinetics;penicillin G acylase; 流化床;固定床;合成;动力学;青霉素酰化酶;
- Keywords penicillin G acylase;constitutive expression system;plasmid stability;glucose; 青霉素G酰化酶;组成型表达系统;质粒稳定性;葡萄糖;
- Keywords Hydrophilic support;Inverse suspension polymerization;Penicillin G acylase;Immobilization; 亲水性载体;反相悬浮聚合;青霉素酰化酶;固定化;
- Keywords penicillin G acylase;layered double hydroxide;immobilized enzyme;glutamic acid intercalated; 青霉素酰化酶;水滑石;固定化酶;谷氨酸插层;
- Synthesis and Characterization of Epoxy-functionalized SBA-15 and Application for the Immobilization of Penicillin G Acylase 环氧基功能化SBA-15介孔分子筛的制备、表征及其固定化青霉素酰化酶
