Partial sequences of the gene were cloned by polymerase chain reaction (PCR) strategy with primers F1 and R1 designed according to the DNA sequence of fae1 reported in Genbank (AF490462).

• 本研究利用GenBank中的fae1基因序列AF490462为模板设计引物,通过多聚酶链式反应(PCR)从白菜、甘蓝和甘蓝型油菜(包括2个人工合成种)的12个不同品种中扩增出长1007bp的fae1基因片段。