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- PCR DNA quantitation PCR DNA 定量法
- PCR DNA quantitation PCR DNA定量法
- Methods: Fluorescence quantitative PCR (FQ - PCR) was used to detect quantitatively UU DNA and CT DNA in randomized in 513 cases NGU. 方法 用荧光定量多聚酶链式反应(FO-PCR)技术对513例非淋患者进行了UU DNA和CT DNA定量检测。
- Methods HBV DNA was detected by quantitative PCR, and HBV-M was detected by ELBA. The mean amount of HBV DNA was counted in different serological marker combinations. 方法 采用定量PCR和定性PCR方法,检测216份不同临床类型血清标本的HBV DNA,再用ELISA方法测定HBV-M,统计不同免疫指标组合的HBV DNA平均含量。
- Method:Serum samples of 106 suspected HBV patients were detected by MEIA and positive samples were then detected for HBV DNA by Fluorescence quantitative PCR. 目的:应用微粒子荧光酶免疫分析法(MEIA)定量检测乙肝病毒e抗原的浓度,以反映乙肝病毒感染的程度。
- After transient transfection into K562 cells, real-time quantitation PCR was used to detect the expression alteration of PCGF4/Bmi-1, and growth curve was utilized to observe the alteration of growth rate of cells. 转染K562细胞,瞬时干扰PCGF4/Bmi-1基因的表达,实时定量PCR检测干扰前后基因表达变化。 利用生长曲线测定干扰载体转染前后细胞生长速度变化。
- Methods The verification of EBE was based on detection of EBV DNA in cerebrospinal fluid (CSF) by fluorogenic quantitative PCR(FQ-PCR) and Nest-PCR. The clinical and CSF changes of 27 EBE cases and 26 controls were analyzed and compared. 方法 对 2 7例经荧光定量PCR(FQ PCR)及巢氏PCR方法检测脑脊液病毒阳性而确诊的EBE与 2 6例单纯疱疹病毒脑炎 (HSE)患儿临床及脑脊液资料进行回顾性分析。
- real time fluorescent quantitation PCR 实时荧光定量PCR
- Conclusion The DNA quantitive analysis with FCM is helpful for the early dignosis of laryngeal carcinoma. 结论流式细胞DNA定量分析有助于喉癌的早期诊断。
- Real-time quantitation PCR assay 荧光定量PCR
- Real-time fluorescent quantitation PCR 实时荧光定量PCR
- Keywords Human parvovirus B19 PCR DNA Microarrray Gene probe; 关键词细小病毒B19;聚合酶链反应;基因芯片;分子探针;
- Fluorescence quantitative PCR combined with RT-PCR technique can detect the DTC in blood of the patients with HCC. RT-PCR联合荧光标记探针杂交法能够检测到肝癌患者血液中播散的肿瘤细胞,并且以肺动脉血中阳性率最高。
- For special aims, multiple PCR, reverse PCR, quantitative PCR, in situ PCR, as well as capillary PCR, were developed. 而适合不同目的的PCR技术也得到了充分的发展,如多重PCR、反转录PCR、定量PCR、原位PCR、PCR突变、毛细管PCR技术等等。
- The expression of RAE-1 and H60 genes was measured by RT-PCR and real-time quantitative PCR. 6) BALB/C小鼠正常肝脏组织能检测到RAE一1低表达。
- Objective To detect Plasmodium falciparum with the Fluorescent Quantitative PCR(FQ PCR) and value this method. 目的评价荧光定量聚合酶链式反应(FQ-PCR)检测恶性疟原虫的效果。
- Objective To evaluate the clinical value of fluorescence quantitative PCR(FQ-PCR) technique in diagnosing tuberculosis. 目的探讨荧光定量聚合酶链反应(FQ-PCR)技术诊断结核病临床应用价值。
- Methods HBV markers and HBV-DNA level in the serum were detected by ELISA and fluorescent quantitated PCR. 方法:通过ELISA法及荧光定量PCR法检测血清标本中HBV标志物和HBV-DNA含量水平。
- Fluorescent real-time quantitative PCR was used to investigate some of the differentially expressed genes. 对部分筛选出来的差异表达基因使用荧光定量PCR方法检测并确认其变化;