PCR assays were developed for the rapid and sensitive detection of virulent factor mrp and epf genes of Streptococcus suis type 2. The sires of the mrp gene and epf gene of S.suis type 2 were a 885 bp and 626 bp PCR products respectively.

• 根据 2型猪链球菌毒力因子溶菌酶释放蛋白 (MRP)与细胞外蛋白因子 (EF)基因 (mrp与 epf)序列 ,分别设计、合成一对引物 ,建立聚合酶链反应 (PCR)方法。