PCR amplified green fluorescent protein (GFP) gene was inserted into pGEX-KG expression vector, and resulting plasmid was designated as pKG-GFP.

• 用PCR扩增出绿色荧光蛋白(GFP)基因，插入到pGEX-KG表达载体中，并将构建出的重组质粒命名为pKG-GFP。