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- Conclusion: The vitrification with EG and DMSO as cryoprotectants could effectively preserve mouse ovarian tissues. 结论:联合应用玻璃化冷冻保护剂EG和DMSO对小鼠卵巢组织学和功能的影响较小。
- AOA was positive which was probably the cause of damage of the ovarian tissues, reduction of sex hormone secretion and impaired fertility. 血清AOA阳性,并导致卵巢组织病理性损伤,性激素产生减少,生育率降低。
- Objective:To explore the effect of programmable freezing on morphology and apoptosis of human primary follicles(PF) and primordial follicles(POF) in human ovarian tissues. 目的:探讨程序化冷冻对人卵巢组织内的始基卵泡与初级卵泡形态与凋亡的影响。
- The expression of DCC gene was lost in no normal ovarian tissues, in 2 (2/10) benign lesions, while the loss of DCC gene expression was found in 19(19/34,56%) carcinomas ( P <0.05 ). 正常卵巢组织中DCC基因表达缺失率为 0%25 ; 卵巢良性肿瘤组织为 2 0%25 ;
- Methods A panel of normal ovarian tissues, primary OC specimens and corresponding LNS was examined for mRNA expression and mutation of MTA1 and nm23H 1 and protin expression of E cad genes by using RT PCR, RT PCR SSCP and immunohistochemistry. 方法 应用 RT- PCR、RT- PCR- SSCP和免疫组化技术 ,对正常卵巢、卵巢癌及其配对淋巴结组织进行 MTA1、nm2 3H1m RNA表达和突变及 E- cad蛋白表达的检测。
- Methods hTERT mRNA expression and telomerase activity were analyzed in 43 samples of EOC,11 benign ovarian tumors and normal ovarian tissues,as well as 4 borderline ovarian tissues with the use of RT-PCR and telomeric repeat amplification protocol(TRAP). 方法 应用RT PCR及端粒重复扩增方法对 43例上皮性卵巢癌、11例良性卵巢肿瘤和卵巢正常组织以及 4例交界性卵巢肿瘤共 69份标本进行hTERTmRNA及端粒酶活性检测。
- The follicles at different development stages in autografts were normal morphology, but the number of follicles in vitrified ovarian tissues was less than that in non-frozen tissues. 移植存活的卵巢组织内可见不同发育阶段的卵泡,形态正常,但冻融卵巢组织内卵泡数量比新鲜组织少。
- The epigynous flower was composed of ovarian tissue, hypanthium and appendicular tissues. 由花器附属组织包围子房形成的子房下位花,于十一月上旬左右绽放。
- This review will focus on the recent progress in human ovarian tissue cryopreservation and transplantation. 本文就国外近年有关人类卵巢组织冷冻、移植的研究进展作一综述。
- But at present the cryopreservation protocols for ovarian tissue is not consummate yet,there are a lot issues unsolved. 但目前卵巢组织的冷冻方案还不完善,有许多问题尚未解决。
- After culture 80% of follicles in normal ovarian tissue were atretic compared with 53% in PCOS biopsies. 培养后正常卵巢组织中 80%25 的卵泡发生闭锁,而 PCOS 卵巢组织中 53%25 卵泡发生闭锁。
- Recently,a lamb was born after orthotopic transplantation of frozen ovine ovarian tissue. 目前,在自体原位移植冻存羊卵巢组织后,已产下一只健康子代羊。
- Conclusions The protocol of slow-freezing/rapid-thawing is suitable for keeping human fetal ovarian tissue. 结论慢速冷冻-快速复苏法可以较好地保存人胎儿卵巢组织超微结构。
- Conclusion The frozen-thawed human fetal ovarian tissue could survived and the follicle developed well when xenografted into the back subcutaneous. 结论冻融人胎儿卵巢组织移植于裸鼠的背部皮下能存活,其卵泡处于良好的生长发育状态。
- They add that studies of the possible presence of HPV DNA in carcinomatous epithelial ovarian tissue or endometrial tissue are conflicting. 癌上皮卵巢组织或子宫内膜组织上可能存在HPVDNA的研究结果不一致。
- This review summarizes the methods and research progress on cryopreservation and transplantation of ovarian tissue in recent years. 本文介绍了卵巢组织冷冻保存和移植的方法及近年来的研究情况。
- So, in theory, women could choose to preserve a sample of ovarian tissue and then reimplant it after their natural fertility had run out. 所以,从理论上讲,妇女能够选择保存卵巢组织,等到她们自然生育能力丧失以后再回植体内是切实可行的。
- Methods: BALB/C female mice model of POF was established by multiple sites subcutaneous injection of ovarian antigen elicited from ovarian tissue of mature SD female rats. 方法:以成熟的SD雌性大鼠卵巢组织制备卵巢抗原,皮下多点注射免疫BALB/C雌性小鼠,建立卵巢早衰模型。
- With the development of cryobiology and reproductive medicine,cryopreservation ovarian tissue becomes another potential choice succeeding to cryopreservation embryos and oocytes. 随着低温生物医学和生殖医学的发展,卵巢组织的冷冻保存成为继胚胎冷冻、卵母细胞冷冻之外保存女性生殖能力又一极具潜力的选择。
- It's more hygienic to use disposable paper tissues. 使用一次性餐巾纸更为卫生。