Our studies the expression of NR1 positive cells with immunohistochemistry, neurons apoptosis with terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end labeling (TUNEL) staining and NO activity,

• 本研究采用免疫组化、原位切口末端标记、放射免疫以及血生化检测的方法来研究在HIBD后腹腔注射rhEPO，海马CA1区神经细胞NR1蛋白的表达变化及神经细胞凋亡，并测定血浆及脑匀浆NO/ET的水平，从而探讨rhEPO在HIBD