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- Keywords Schwann cells;Cell culture;One enzyme digestion method;Rat; 关键词施万细胞;细胞培养;单酶消化法;大鼠;
- One enzyme digestion method 单酶消化法
- Conclusion: The enzyme digestion method can effectively isolate mesenchymal stem cells with a high proliferative ability and multipotent differentiation ability. 结论：酶消化法可有效的从胎盘组织中分离间充质干细胞，增殖能力强，具备多向分化能力。
- Two fragments of genomic and mitochodrial DNAs were amplified by polymerase chain reaction (PCR). The mtDNA A1555G mutation was identified by the Prev-DAF Testing Kit and the GJB2 235delC mutation was detected by Apa I restriction enzyme digestion method. 血样经DNA提取,进行GJB2 235delC突变、线粒体DNA 12SrRNA A1555G突变的检测。
- Methods By tryosin and DNA enzyme digest methods, we observed the human beings chorial trophcytes with light microscope and electronic microscope. 方法应用胰蛋白酶／DNA酶消化法进行消化，经光镜和电镜观察。
- Nucleic acid enzymatic digestion method 核酸酶消化法
- The enzyme digestion procedure is a stable and reliable method to obtain bovine retinal pericytes. 酶消化法原代培养牛视网膜周细胞是一种稳定、可靠的方法。
- The Prev-DAF Testing Kit was used to detect mtDNA A1555G mutation.The GJB2 235delC mutation was identified by restricted enzyme Apa I digestion method. 为减少社会和家庭的负担，进行聋病的分子流行病学调查显得非常重要，根据调查结果可以正确指导产前检查，从而减少聋儿的出生率。
- Epithelial depuration was carried out by integration of burning and enzyme digestion. 细胞形态观察和电镜检测鉴定细胞。
- These results suggest that the presence of ethionine might have caused the alteration of more than one enzyme in the biosynthesis of amino acids in the selected calluses. 说明在所筛选愈伤组织中存在一个以上与氨基酸合成相关的酶发生改变。
- Both enzyme digestion and alkali treatment are effective methods of cell rupture, but each has a major disadvantage. 酶消化法和碱处理法都是细胞破碎的有效方法，但是也都有各自的缺点。
- Results: Recombinant expression plasmid of ALR was confirmed correct by restriction enzyme digestion and sequencing. 结果：酶切鉴定及测序结果提示表达产物正确；
- The obtained bands were cloned into pGEM T-Easy vector, and the positive clone were confirmed by EcoR I enzyme digestion. 将所获片段克隆入pGEN T-Easy载体，EcoR I酶切鉴定。
- The recombination vector that was named as pCAM-P was obtained based on the tests of PCR and enzyme digestion of the vector. 2. PCR检测、质粒酶切分析结果表明获得了Pto与pCAMBIA1300的重组子，命名为pCAM-P。
- The purpose of the study is to compare the traditional acid method. incineration method and microwave digestion method. 本文比较了三种样品消化方式，即传统酸消化法、灰化法及微波消化法。
- SCs were isolated by warm enzyme digestion, tissue culture, cold enzyme digestion of substep and cold enzyme digestion of combine. 雪旺细胞的分离采用了组织块法、热酶消化法、冷酶分步消化法和冷酶联合消化法。
- The direct comression and artificial digestion method were used to examine the encysted metacercaria of C. 用直接压片法和消化法检查淡水鱼虾体内的华支睾吸虫囊蚴。
- The cells were cultured by explant culture and enzymatic digestion. 组织化学染色和异种移植。
- Restriction enzyme digestion and AT clone were employed to rapidly screen out the recons of PA segments for the preparation of the gene chip probes. 利用酶切、AT克隆方法快速分析筛选出保护性抗原基因片段的重组子,从而制备成芯片探针。
- BACKGROUND: At present, the preparation methods of acellular vascular matrix include enzyme digestion and decontaminant. 背景：目前脱细胞血管基质的制备方法有酶消化法和去污剂法。
