Objectives: To explore the functional property of fusion protein encoded by pGJA-P/VAX1. Methods: COS-7 cells were transfected with pGJA-P/VAX1 or pVAX1 using sofastTM transfect reagent.
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- 目的:体外检测靶向融合防龋DNA疫苗pGJA-P/VAX1编码蛋白的生物学活性,研究蛋白是否具有靶向于抗原递呈细胞如树突状细胞的能力。 方法:pGJA-P/VAX1和pVAX1分别转染真核细胞COS-7,收集并浓缩细胞培养上清。
