Objective:To establish a reversed-phase high performance liquid chromatography(HPLC) method for the determination of paracetamol/tramadol and its metabolite O-desmethyltramadol(M1)in human plasma.

• 目的:建立一种用高效液相色谱法(HPLC)同时测定人血浆中对乙酰氨基酚/曲马朵及其代谢物氧去甲基曲马朵(M1)浓度的方法。