Objective To study on the expression of His-Tagged S protein of hepatitis B virus in Pichia pastoris and its application in combination with the blood-origin hepatitis B virus Dane particles for immunoassay of HBsAb.

• 目的用DNA重组技术在毕赤酵母中表达多聚组氨酸融合重组乙型肝炎病毒表面抗原S蛋白,与血源乙型肝炎Dane颗粒联合使用,制备乙型肝炎表面抗体酶免试剂盒。