Objective To establish the method for extracting the cytokeratin filament aggregating protein (filaggrin) from human epidermis,to detect the anti filaggrin antibody (AFA) in rheumatoid arthritis (RA) and to determine the diagnostic value of AFA in RA.

• 目的 建立从人表皮提取聚角蛋白微丝蛋白 (filaggrin)抗原的方法 ,检测抗filaggrin抗体 (AFA)在类风湿关节炎 (RA)中的分布 ,探讨AFA对早期RA诊断的价值。