OBJECTIVE: To establish a high performance liquid chromatographic (HPLC) method for determining two major metabolites of caffeine, 5 acetylamino 6 formylamino 3 methyluracil (AFMU) and 1 methylxanthine (1X).

• 目的：建立一套测定咖啡因的2种主要代谢物AFMU和1X的高效液相色谱（HPLC）方法。