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- METHODS Gradient elution HPLC was used to analyze the alcohol extracts of Radix Salviae Miltiorrhizae. 方法采用梯度洗脱高效液相色谱法分析丹参乙醇提取物。
- The HPLC was subjected to gradient elution and negtive ion mode was adopted in mass spectrum. 高效液相色谱用梯度洗脱,质谱用负离子模式。
- The extracted phenols were detected by an ODS column, gradient elution and programmable UV wavelength detector. 实验以ODS柱为分离柱，采用梯度洗脱，可编程紫外检测器进行样品检测。
- The effective and active components in bamboo leaves were separated using gradient elution and column chromatography. 采用梯度洗脱及柱色谱法对竹叶中的有效活性成分重点为黄酮进行了分离研究、提纯。
- In order to get better resolution, a simple device which can produce gradient elution is designed. 为了达到更好的分离效果,我们参考文献上有关于梯度冲堤的方法,设计出一个简单的装置。
- A simple gradient elution device and a UV-Vis spectrophotometric detector for HPLC was de, signed. 设计了一种用于高效液相色谱分析的简易梯度洗提装置及UV分光光度检测器。
- Microcystins were successfully separated under isocratic and gradient elution,and the latter has better efficiency. 在等度及梯度洗脱条件下,三种毒素均被完全分离,梯度洗脱时峰形更好,检测灵敏度较高。
- Gradient elution HPLC was used to analyze methanol extracts from white muscardine silkworm,and analyzed the data of their fingerprints by similarity. 采用梯度洗脱高效液相色谱法对获取的指纹图谱数据进行相似度分析,建立了白僵蚕的指纹图谱。
- METHODS Gradient elution method was used to determine the content of andrographolide and dehydroandrographolide in Qinghuo Zhimai tablets. 方法:用梯度洗脱法测定清火栀麦片中穿心莲内酯和脱水穿心莲内酯的含量。
- The kinetic data was measured with a non gradient reactor, The parameters of the model equation were calculated based on experimental results by the Powell non linear regression method: k 1=0 152e -62073RT , k 2=8.18e -2384RT ,k 3=0.221e -18949RT . 采用内循环无梯度反应器测定了SO2 氧化反应动力学数据 ;并利用 Powell法对动力学模型进行参数估值 ;得到 :k1=0 .;15 2 exp(- 6 2 0 73/ RT);k2 =8
- A novel method for the fast optimization of binary multi-step gradient elution in reversed phase-high performance liquid chromatography was proposed. 提出了一种反相高效液相色谱中二元多台阶梯度分离条件快速优化方法。
- Dual linear gradient elution can be performed with two specially made glass container and a high pressure constant flow pump. 用两个特制的玻璃容器及一台平流泵即可实现二元线性梯度洗提。
- In this way, the different grades of acetonitrile and methanol were tested to show the feasibility with which they are applied in gradient elution. 按照此法，对国产不同等级的乙腈和甲醇进行了测试，指明这些试剂用于梯度洗脱的可能性。
- Gradient elution HPLC was used to analyze methanol extracts from white muscardine silkworm, and analyzed the data of their fingerprints by similarity. 采用梯度洗脱高效液相色谱法对获取的指纹图谱数据进行相似度分析，建立了白僵蚕的指纹图谱。
- Objective:To study on chromatography fingerprint(CFP) of Huangqi herbs.Method: HPLC was used on a Hypersil ODS column with water-acetonitriles gradient elution. 应用高效液相色谱法研究了黄芪的指纹图谱,并对毛蕊异黄酮、芒柄花素及黄芪甲苷进行了含量测定。
- Shim-pack VP-ODS is used as column, ACN: EtOAc(21:3, V/V) as mobile B, and aqueous acetic acid (50:1,V/V) as mobile A, linear gradient elution is adopted. 流动相A为2%25醋酸(HAc)，流动相B为乙腈(ACN)； 乙酸乙酯(EtOAc)=21：3(v/v)，梯度洗脱；
- Methods: Chromatographic fingerprint of Coleus forskohlii was investigated by HPLC-ELSD and gradient elution mode was applied to chromatographic separation. 方法:采用HPLC-ELSD法,梯度洗脱,研究毛喉鞘蕊花药材的指纹图谱。
- Method The main ingredient of the injection, Red Ginseng, was determined by HPLC and used binary solvent-delivery gradient elution to control the main component in Red Ginseng. 方法采用HPLC法,以君药红参中人参皂苷为检测指标,采用梯度洗脱的方法,控制其中红参主要成分的色谱峰。
- Methods Artemisinin is extracted with petroleum ether and pass column of silica gel and gradient elution with ethyl acetate,then it is tracked with TLC and detected with UV-VIS. 方法青蒿经石油醚提取浓缩,浸膏经过硅胶柱,用不同配比的乙酸乙酯洗脱,用TLC法跟踪,UV法检测。
- Two dioscin products and aglycone from enzyme reaction are separated and purified by silica gel column gradient elution with the solvent of chloroform and methanol. 摘要用柱层析法对穿山龙薯蓣皂苷酶解产物进行分离纯化（硅胶柱中以氯仿甲醇洗脱），得到2种单体皂苷和皂苷元。
