Neither of the other two NLSs (NLS2 and 3) was functional when expressed alone, but exhibited similar activity to NLS1 when they were expressed as a fusion peptide.

• NLS2 和3 单独融合荧光蛋白表达，荧光信号在细胞内呈分散式的分布，但如果NLS2 和3 作为一个肽段与荧光蛋白融合表达，大部分荧光信号也只出现在细胞核内。