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- Treat the MOLT-4 cells with anti-HCV antibody and HCV RNA positive and negative serum, respectively. 用抗HCV抗体和HCVRNA阳性及阴性血清感染MOLT-4细胞,制备细胞片进行免疫酶染色。
- Methods Compare the effect of antisperm 60KD?78KD antibody positive serum and antisperm antibody negative serum on the sperm in vitro. 方法使用抗60KD和抗78KD阳性血清及抗精子抗体阴性血清体外处理精子,比较它们对精子的凝集、细胞毒、穿透高度和顶体反应的影响。
- Negative serum , positive serum (its agar gel precipitation
titer is 1: 16), rabbit-duck serum (its AGP titer is 1: 128), HRP enzyme-labeled rabbit-duck IgG were also produced. 制备了阳性血清(琼扩效价为1:16)、阴性血清、二抗(兔抗番鸭)阳性血清(琼扩效价为1:128),提纯二抗并标记HRP酶,获得合格的酶标二抗。 建立了间接ELISA检测方法:抗原稀释30倍,酶标二抗稀释50倍,血清稀释50倍,临界值为0.;5。 - Objective To obtain the antigenic epitopes which can affinitive bind to theHCV(hepatitis c virus) polyclonal antibodies by using a phage 12-mer peptide libraryand to test the efficiency of converse biopannning by using negative serum. 目的筛选噬菌体展示随机12 肽库中可以与HCV 抗体阳性的血清特异性结合的序列,在HCV 表达的蛋白分子上找到这个抗原表位, 分析其序列、结构特点与功能的关系。 同时检验用阴性血清逆向筛选是否能提高筛选效率。
- Methods:Applying TP-ELISA,TRUST and colloidal gold test to test 102 positive sera,220 negative sera and 5 indeterminable sera were tested by TPPA,respectively. 方法:分别用TP-ELISA、TRUST、金标法对102份TPPA确证阳性血清;5份TPPA可疑血清;220份TPPA确证阴性血清进行实验;比较3种梅毒初筛方法的敏感度和特异度。结果:TP-ELISA敏感度98.;04%25;特异度100%25;
- The doctor inoculated her with the serum. 我们所有人都注射了天花病毒。
- The answer to my request was in the negative. 对我的请求的答复是否定的。
- His answer to my request was a negative. 我的请求遭到了他的拒绝。
- The tests for hepatitis were negative. 肝炎检查结果是阴性。
- Their criticism was not negative. 他们的批评并不是负面的。
- Do a blow-up of this corner of the negative. (对某事的)详尽的报道。
- Relating to bodily fluids, especially serum. 体液的与体液(尤是血清)有关的
- Serum AFP determination was done in 10 cases with all negative. 10例血清甲胎蛋白测定全为阴性。
- I have a negative opinion on the value of this idea. 我对这个主意持反对意见。
- coli BL21. In Western-blot assay, the fusion proteins reacted with 10 of 12 (83. 3%) HHV-6 IgM-positive serum samples and none of the 8 negative serum samples. coli BL21中有效表达,表达产物为硫氧还(HP-thioredoxin)融合蛋白,纯化后获得单一目的蛋白条带。 Western-blot检测结果表明该融合蛋白可与12份HHV-6 IgM阳性血清中的10份(83.;3%25)发生反应,而与8份阴性血清均不发生反应。
- How many copies do you ask us to print off from the negative? "用那张底片,你要我们印几张像片?"
- A way of producing the negative of a number by obtaining its complement. 一种通过求一个数的补码来得到该数的负数的方法。
- The positive or negative character of a proposition. 命题的性质命题的肯定或否定的性质
- He suffered from serum intoxication. 他得了血清中毒。
- One profile was obtained for whole serum. 其中一个图谱是为全血清作出的。