Mothods:Cerebral cortex was dissected out from E14 embryonic mice and made into single cell suspension,, which cultured by the technique of serum free cultivation combined with growth factors (EGF and bFGF).

• 方法:将孕14天小鼠引颈处死,分离取出胚鼠大脑皮层,制成单细胞悬液,采用无血清培养法,在表皮生长因子(EGF)和碱性成纤维生长因子(bFGF)作用下培养,观察不同阶段的神经干细胞生长情况。