Morphological, physiological and biochemical markers of rejuvenated shoots with higher rooting capability developed from in vitro long-term subculture were identified.

• 借鉴试管复幼诱导生根的经验，通过芽接或试管继代培养，获得幼化材料，再进行埋干处理，成功地诱导辽宁1号核桃品种嫩枝扦插生根。