More hydrophilic peptide could be eluted by polarer solvents efficiently.Its recovery rate could be improved by increasing trifluoroacetic acid wash times.

 
  • 结果表明,洗脱溶剂对多肽的洗脱效果与多肽及溶剂的极性有关:亲水性较强的多肽用极性较强的溶剂洗脱时效果较好,可用增加三氟乙酸的洗脱次数来提高这类多肽的洗脱率;
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