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- The methylation specific PCR (MSP) and bisulfite DNA sequencing were performed to examine the methylation status of SLIT2 gene promoter. 亚硫酸氢钠处理DNA,用MSP和测序法检测SLIT2启动子甲基化状态。
- Methods The methylation status of CpG island of AR gene promoter in 3 leukemic cell lines and 15 leukemic bone marrow cells was detected by methylation specific PCR(MSP). 方法应用甲基化特异性PCR技术(methylation specific PCR,MSP)检测3种白血病细胞株及15例患者骨髓标本雄激素受体基因甲基化状态。
- Using methylation specific PCR (MSP) to detect the CpG island methylation changes of PRA & PRB promoters in the leukemia cell lines before and after 5-Aza CdR treatments and the MG88 transfection. 利用MSP检测白血病细胞株在5-Aza CdR处理前后、MG88的转染前后PRA、PRB启动子CpG岛甲基化状态。
- Keywords Lung cancer;Epithelial Cadherin;Methylation specific PCR; 肺癌;上皮钙粘蛋白;甲基化特异性PCR;
- The influence on clinical values by optimizing the annealing-temperature of methylation specific PCR 改变退火温度对甲基特异性PCR检验指标有效性的影响
- Study on the methylation of p15 gene CpG islands in acute leukemia: using methylation specific PCR method 采用甲基化特异性聚合酶链反应研究急性白血病p15基因CpG岛甲基化
- Keywords DNA methylation;epigenetics;adenomatous polyposis coli （APC）;lung cancer;methylation specific PCR （MSP）;quality control; 关键词DNA甲基化;表观遗传;APC基因;肺癌;甲基化特异性PCR;质量控制;
- methylation specific PCR 甲基化特异性PCR
- The sensitive and specific PCR method for detection E. granulosus from dog faeces was preliminarily developed. 初步建立了灵敏、特异的检测家犬粪便中细粒棘球绦虫的PCR方法。
- Methylation Specific Polymerase Chain Reaction (MSP) was used to test the CpG island methylation status of cancer related genes P16, MGMT and hMLH1. 应用甲基化特异性PCR法检测癌组织、边缘组织和正常食管粘膜组织中肿瘤相关基因P16、MGMT和hMLH1启动子区甲基化状态，分析DNA异常甲基化是否与食管癌临床特征和环境因素间存在联系，并探讨食管癌相关基因异常甲基化与叶酸代谢酶基因MTHFR C677T多态间的关系。
- Method Several pairs of specific PCR primers were designe d according to the conserved region of HDV genome. 方法针对HDV基因保守区域设计多对PCR引物。用芯片点样仪将PCR产物点到玻片上制成基因芯片。
- Conclusion The sensitive and specific PCR method for detection E. granulosus from dog faeces was preliminarily developed. 结论初步建立了灵敏、特异的检测家犬粪便中细粒棘球绦虫的PCR方法。
- Methods The specific PCR primers were designed and synthesized to amplify the DNA fragments enconding preS1/S2/S antigen gene from the plasmid pHBV adr by PCR method. 方法设计合成2对寡核苷酸引物,以adr亚型HBV质粒pHBV DNA为模板,采用PCR法分别扩增HBV大分子表面蛋白基因(preS1/S2/S基因)片段;
- The established specific PCR assay should provide a practical and rapid method for the identification and differentiation of Eimeria species in mixed infections. 用本项研究所建立的特异PCR方法对这些混合感染的球虫种类进行鉴定具有一定的实用价值。
- Objective:Evaluation of methylation specific polymerase chain reaction and its potential clinical value in the detection of minimal residual disease (MRD) in adult acute leukemia with P15. 目的:评价由甲基化特异性聚合酶链反应分析P15基因甲基化对成人急性白血病微小残留病灶的检测价值。
- We genotyped some main Chinese sweet cherry cultivars for the first time using the alleles specific PCR, and two new S genotype (S1S6, S4S9) were found, which made the listing of S-allele assignment more completed. 对57个国内外的甜樱桃品种进行了自交不亲和(SI)基因型的鉴定，首次鉴定了我国主栽的一些甜樱桃品种的S基因型，并发现了两个新的S基因型S1S6、S4S9，将自交不亲和的组群扩展到21个。
- Conclusion The SNP fluorescent-multiplex system based on the fragment length discrepant allele specific PCR strategy is simple and economical, and is of a high application value in forensic medicine. 结论采用荧光标记复合扩增毛细管电泳法进行SNP分型，方法简单实用，在法医学个人识别领域具有较高的应用。
- Methylation specific polymerase chain reaction 甲基化特异性聚合酶链反应
- nested methylation specific polymerase chain reaction 巢式甲基化特异性聚合酶链反应
- Education should not be restricted to any one specific age group. 教育不应限制在任何特定的年龄组上。
