MethodsThe supporter paper fiber was activated with epoxy chloropropane etc.According to the influence of spacer and different ligand on absorption of kinase,process route was optimized.

• 方法以纸纤维为载体,环氧氯丙烷等为活化剂,考察接臂、配基对磷酸激酶吸附效果的影响,确定亲和色谱介质制备的工艺路线,并对主要工艺参数进行优化。