MethodsCytospin of 12 fresh vitreous specimens of PVR grade B,18 ERM specimens of PVR C and D were prepared and stained by TUNEL method and immunohistochemical technique respectively for the detections of apoptosis and bak and bcl-xl.

• 方法 对 12例PVRB级玻璃体切割液离心收集物 ,18例C级以上PVR的ERM进行研究 ,末端标记 (TUNEL)染色法检测凋亡细胞 ,免疫组织化学染色法检测bak和bcl xl基因的表达。