Methods:Two epidemic strains 02k38 and 05k44 were isolated from infants or babies with infection of human rotavirus in epidemic session in Kunming district in 2002 and 2005. The full-length NSP4 genes were amplified by RT-PCR and cDNA was sequenced.

• 方法从昆明地区2002年和2005年秋冬季婴幼儿腹泻流行期不同程度腹泻患儿中分离得到两株轮状病毒流行株02k38和05k44,RT-PCR扩增NSP4全长基因,进行cDNA序列测序。