Methods:Total mRNA of BEL7402 cells was extracted. The RT PCR products of AFP mRNA were purified and combined with PMD 18T vectors.

• 方法 :提取肝瘤细胞株BEL740 2细胞总RNA ,进行RT PCR扩增并纯化AFP基因片段 ,与PMD 1 8T载体连接构建重组质粒。