Methods:To detect mRNA levels of bcr-abl fusion gene of 36 patients with CML by RQ -PCR and nested RT-PCR ,Ph chromosome using cytogenetic study.

• 方法:采用实时定量逆转录聚合酶链反应(Real-time quantitative RT-PCR,RQ-PCR)及巢式RT-PCR方法检测36例CML患者bcr-abl mRNA; 并用细胞遗传学方法检测Ph染色体。