Methods:The immunogen, amoxicillin-BSA, was synthesized by carbodimide method;the coating antigen, amoxicillin-OVA, was synthesized by isobutylchloroformate method.

• 方法:采用碳二亚胺两步法将半抗原阿莫西林与牛血清白蛋白偶联制备免疫原,采用混合酸酐法将阿莫西林与卵清蛋白偶联制备ELISA包被原。