Methods:Red blood cell(RBC) was isolated from the anti-coagulated fresh pig blood,It was hemolyzed by heated water,then heme was extracted with 3% acetone-hydrochloric acid.

• 方法:采用新鲜猪血抗凝离心后取血球,水浴加热溶血后,用3%25盐酸丙酮提取血红素,提取液加鞣酸/氯化锶沉淀分离出血红素。