Methods:RT-nested PCR and direct immunofluorescence assay(FA) were adopted to determine HFRS antigens in the lung tissues of captured rats by surveillance on natural infection of rodents with hantavirus in Shenzhen city in 2005,respectively.

• 方法:用RT-nested-PCR和直接免疫荧光法(direct immunofluorescence,FA)分别检测深圳市2005年肾综合征出血热宿主动物监测中捕获的鼠类中HV的感染情况。