Methods:Naive OVA-TCR-Tg CD4~+T cells were isolated from spleens and lymph nodes of DO11.10 mice. The cells were labeled with CFSE in vitro and then were adoptively transferred into normal mice. One day after transfer,mice were immunized i.v.

• 方法:自CD4+T细胞受体转基因(TCR-Tg)小鼠脾和淋巴结分离CD4+T细胞,体外利用CFSE进行标记,然后被动输给相同基因的正常小鼠。