Methods:NMPs were prepared by high salt extraction and immunized rabbit to raise polyclonal antibody which was used to detect the membrance transferred from 2 D gel of nuclear matrix in normal WBC and acute myeloid leukemia cells by Western blot.

 
  • 方法 :高盐抽提法制备白血病细胞NMPs ,免疫动物制备多克隆抗体 ,以此作为免疫学探针对正常WBC和白血病细胞NMPs进行双向电泳 ( 2DE)_免疫印迹 (Westernblot)检测。
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