Methods:First,purifying the enzyme by precipitation with ammonium sulfate,then through CM Sepharose Fast Flow chromatography.The purity and the molecular weight was detected by the SDS-PAGE.

• 方法:先用硫酸铵沉淀,然后通过羧甲基琼脂糖快速胶柱层析后进行分离纯化,并用SDS-PAGE测定纯度、相对分子质量,及其对人血凝块的水解活性。