Methods: Using doubling immuno-colloidal gold labeling of anti-tryptase(AA5)and anti-chymase(CC4),the ultrastructure of secretory granule in mast cell was observed by transmission electron microscope.

• 方法:用双重免疫胶体金标记类胰蛋白酶抗体(AA5)及类糜蛋白酶抗体(CC4),在透射电子显微镜下观察肥大细胞内分泌颗粒的超微结构。