Methods: The cells were cultured under routine conditions using Dulbecco?s modified Eagle?s medium (DMEM) without L Arginine(L Arg).

• 方法:人肝癌细胞Bel-7402按常规条件培养于含10%25小牛血清且无L-精氨酸(L-Arg)的DMEM培养液中。