Methods: The TK gene was transfected into human gastric carcinoma cell line MKN28 using HSV-TK that packed with PA317 cell, the sensitivity of MKN28TK cells to GCV was examined in vitro.

• 方法：应用PA317细胞包装的逆转录病毒介导的HSV-TK基因系统，在体外以逆转录病毒上清感染法将TK基因导入MKN28人胃癌细胞，并初步观察更昔洛韦（GCV）对转染TK基因的MKN28胃癌细胞的杀伤作用。