Methods: The CB-HRP/ Fos double-labeling method was used to observe the expression of Fos in CB-HRP positive neurons following the sigmoid pain stimulation.

• 方法:采用CB-HRP/Fos双重标记技术,观察乙状结肠痛大鼠远位触液神经元中Fos样免疫反应蛋白(Fos)表达的变化。