Methods: The ADM content in myocardia and aortas were measured in SHRs and male Wistar Kyoto (WKY) rats (11-week-old) by radioimmunoassay (RIA). The mRNA levels of ADM, CRLR, RAMP2 and RAMP3 were determined by semi-quantitative RT-PCR.

• 方法通过放射免疫法测定SHR和WKY大鼠心肌和血管ADM含量,半定量RT-PCR测定ADM、CRLR、RAMP2和RAMP3 mRNA水平。