Methods: Terminal deoxynucleotidyl transferase mediated dUTP-biotin nick endlabeling(TUNEL staining method) ,bcl-2 and bax immunohistochem-isty method were used to observe apoptosis and the expression of bcl-2 and bax genes.

• 方法:采用原位末端标记法(TUNEL染色法)及免疫组织化学方法,对生后小鼠肾脏髓质发育中的细胞凋亡、Bcl-2和Bax基因蛋白表达进行观察。